LSR II and FACSAria IIIu Dye Selection Guide
Choose only one dye for each PMT.
Please discuss your choices with flow core personel as not all combinations are workable.
| Excitation Laser | Detector | Bandpass Filter |
Filter nm | Dye |
|---|---|---|---|---|
| Violet 405 nm | PMT A | 740 LP | 780/60 | |
| PMT B | 670 LP | 705/70 | ||
| PMT C | 630 LP | 640/40 | ||
| PMT D | 595 LP | 605/40 | ||
| PMT E | 505 LP | 525/50 | ||
| PMT F | 450/50 | |||
| Blue 488 nm | FSC Diode | 488/10 | Forward Scatter | |
| PMT A | 685 LP | 695/40 | ||
| PMT B | 505 LP | 530/10 | ||
| PMT C | 488/10 | Side Scatter | ||
| Green 552 nm | PMT A | 780/40 | PE-Cy7 | |
| PMT B | 685 LP | 710/50 | PE-Cy5.5 | |
| PMT C | 635 LP | 670/30 | ||
| PMT D | 600 LP | 610/20 | ||
| PMT E | 575/26 | |||
| Red 637 nm | PMT A | 750 LP | 780/60 | |
| PMT B | 685 LP | 710/50 | ||
| PMT C | 660/20 | |||