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Guidelines for Submitting 10x Samples to the Genomics Core

Samples for Library Preparation

Submission for 10x Chromium X

Single Cell 3' Expression Library

Please contact the Genomics Core 2 weeks prior to the initiation of your single  cell expression project. Researchers must coordinate their sample delivery with core personnel prior to submission. The cell sample must be submitted as a completely dissassociated cell suspension with a high viability percentage of >80%. Please use the following link to access the best practices for sample preparation and access to supporting documents and training videos for cell preparation and single cell expression project planning.

Submission Guidelines 

Single Cell 5' Expression Library and V(D)J Enrichment Library

Please contact the Genomics Core 2 weeks prior to the initiation of your single cell expression project. Researchers must coordinate their sample delivery with core personnel prior to submission. The cell sample must be submitted as a completely dissassociated cell suspension with a high viability percentage of >80%. Please use the following link to access the best practices for sample preparation and access to supporting documents and training videos for cell preparation and single cell expression project planning.

Submission Guidelines

Single Cell ATAC Chromatin Library

The success of a single cell ATAC sequencing project is determined by the quality of the cell nuclei preparation provided to Genomics Core. Researchers must coordinate their sample delivery with core personnel at least 2 weeks prior to submission. The nuclei sample must be submitted as a completely dissassociated nuclei suspension derived from cells with a high viability percentage  >80%. Cryopreserved cells are acceptable for nuclei isolation. The 1X Nuclei Buffer required for nuclei resuspension will be provided by the Genomics Core personnel to support your Single Cell ATAC project.

Submission Guidelines

Single Cell Multiome ATAC + Gene Expression

Single cell multiome ATAC + gene expression requires cell nuclei to initiate the multiome library preparation. The success of a single cell multiome ATAC + gene expression sequencing project is determined by the quality of the cell nuclei preparation provided to the Genomics Core. Researchers must coordinate there sample delivery with core personnel 2 weeks prior to submission. The nuclei sample must be submitted as a completely dissassociated nuclei suspension derived from cells with a high viability percentage > 80%. Cryopreserved cells are acceptable for nuclei isolation. The 1X Nuclei Buffer required for nuclei resuspension will be provided by the Genomics Core personnel to support your single cell multiome ATAC + Gene Expression project.

Submission Guidelines

Chromium Gene Expression Flex - Fixed RNA

Chromium Gene Expression Flex - Fixed RNA single cell can be performed using fresh, frozen and FFPE fixed samples. Singulated cells are required to initiate the protocol. Tissue or cells can undergo a fixation protocol allowing for -80C storage prior to the Chromium processing of the singulated cells or nuclei allowing the workflow to be optimized for multiple sample management or time course studies. Samples can be batched with multiplex barcoding. Researchers must coordinate there sample delivery with core personnel 2 weeks prior to submission. The cells or nuclei must be submitted as a completely dissassociated suspension.

Submission Guidelines

Sample Submission

10x Chromium X Libraries

Please contact Genomics Core personnel to coordinate project planning and your sample delivery. The subsequent sample delivery address is listed below.

Attn: Clark Bloomer
Genomics Core
1015 Hemenway
University Receiving Dock
2106 Olathe Blvd.
Kansas City, KS 66160

For further information on the Genomics Core please browse the rest of our website. Please feel free to call if you have any questions or require directions to the facility.

Genomics Core Contacts

Clark Bloomer, Project Manager - cbloomer@kumc.edu  (913) 588-7127
Rosanne Skinner, Sequencing Specialist - rskinner@kumc.edu  (913) 588-6927
Veronica Cloud, Ph.D., Sequencing Specialist – vcloud@kumc.edu 913-588-9564

Genome Sequencing Facility

University of Kansas Medical Center
Genome Sequencing Facility
1015 HLSIC MS 3028
2146 West 39th Street
Kansas City, KS 66160-7421
Phone: 913-588-7127
Fax: 913-588-7131