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Pricing

NovaSeq 6000

The following pricing for NovaSeq 6000 v1.5 sequencing includes initial sample QC*, library preparation, library validation and qPCR quantification at the designated read count or X coverage. Read count and X coverage can be adjusted in 5M read or 5X increments. (*Additional sample QC subject to charge)

The KIDDRC Bioinformatics Core on the University of Kansas Medical Center Campus is available to provide sequence data analysis services. Please contact Sumedha Gunewardena sgunewardena@kumc.edu for service information.

SP Flow Cell  -  S1 Flow Cell  -  S2 Flow Cell  Pricing

Service KUMC/KU, KS Regents, Frontiers Investigators Non-Affiliated Academic Investigators

SP

S1

S2

SP

S1

S2

Tecan Universal Plus mRNA
25M reads
2x100bp 

$290

$270

$250

$365

$340 

$315

Illumina Stranded Total RNA
40M reads
2x100bp

$450

$420

$385

$565

$525

$485

Illumina RNA Prep + Enrichment – Human, (L) Tagmentation
25M reads
2x100bp

$475

$455

$440

$600

$570

$550

Illumina DNA Prep with Enrichment - Human Exome
50M reads / 50X - (4Gb)
2x100bp

$460

$420

$380

$575

$525

$475

Other Exome Captures

Inquire

Inquire

Inquire

Inquire

Inquire

Inquire

TruSeq ChIP
30M reads
2x50bp (SP) / 2x100bp (S1/S2)

$405

$385

$360

$510

$485

$450

Additional 5M read increments

$22

$18

$14

$28

$23

$18

Illumina DNA Prep - Human Genome
30X coverage
2x150bp

$1915

$1585

$1155

$2395

$1985

$1445

Ovation RRBS Methyl-seq w/ oxBS Module:
Human - 55M Reads
2x100bp

$500

$455

$415

$625

$570

$520

Other Genomes

Inquire

Inquire

Inquire

Inquire

Inquire

Inquire

Additional X Coverage

Inquire

Inquire

Inquire

Inquire

Inquire

Inquire

 


S4 Flow Cell Pricing

Service KUMC / KU, KS Regents, Frontiers Investigators Non-Affiliated Academic Investigators

Illumina DNA Prep - Human Genome
30X coverage
2x150bp

$715 $895

Ovation RRBS Methyl-seq w/oxBS:
Human - 55M Reads
2x100bp

$350 $440

Other Genomes

Inquire Inquire

Additional X Coverage

Inquire Inquire

For Full Flow Cell Run Pricing please direct inquiries to:  cbloomer@kumc.edu  or call 913-588-7127


Sequence Library Preparation (see descriptions below)

Service Description KUMC/KU, KS Regents, Frontiers Investigators Non-Affiliated Academic Investigators
Cost/sample
Library prep. includes initial sample QC,
library validation,
qPCR quantification.
Additional sample QC
subject to charge.
Tecan Universal Plus mRNA $180 $198
Illumina Stranded Total RNA

$274

$301
Illumina RNA Prep + Enrichment - Human $372 $409
miRNA Library Prep $206 $227
Illumina DNA Prep $145 $160
TruSeq ChIP-Seq $276 $304
Illumina DNA Prep - Human Exome $239 $263
Ovation RRBS Methyl-seq w/ oxBS $268 $295
Library Pooling / Indexing Fee $11 $12
Library Quant. (qPCR) $21 $23

Library Description

Tecan Universal Plus mRNA-seq Library w/ UDI – Employs oligo dT enrichment of messenger RNA to assess expression including alternative transcripts and gene fusions. Provides strand of origin to detect antisense transcription. Unique Dual Index (UDI) adapter compatible.

  • Input range of total RNA 10ng – 1ug. Targeted input 1ug.
  • Sequencing recommendation – 25M 100bp paired end reads*.

Illumina Stranded Total RNA Library – Includes Ribosomal reduction to enrich for mRNA and non-coding transcript interrogation. Provides strand of origin to detect antisense transcription. Compatible with FFPE RNA. Unique Dual Index (UDI) adapter compatible.

  • Input range of total RNA 100ng – 1ug. Targeted input 1ug.
  • Sequencing recommendation – 40M 100bp paired end reads*.

Illumina RNA Prep + Enrichment, (L) Tagmentation Library – Couples reverse transcription of RNA to dscDNA and On-Bead Tagmentation with an enrichment panel (Illumina Exome Panel or Respiratory Virus Oligo Panel v2 w/ SARS-CoV-19) to capture coding transcriptome/RNA exome.  Compatible with low yield, low quality and FFPE total RNA. Targeted input variable based on RNA quality. Unique Dual Index (UDI) compatible.

  • Targeted input of total RNA 10ng - 100ng. Input range of low-quality RNA or FFPE RNA 20ng – 100ng (%DV200 ≥ 36.5%).
  • Sequencing recommendation – 25M 100bp paired end reads*.

miRNA Library – Enables library preparation of any miRNA with a 3’ hydroxyl and a 5’ monophosphate present from Dicer/Drosha processing. Used to find novel miRNAs, characterize variation with single base resolution and analyze differential expression.

  • Targeted input – 1ug total RNA or 10 - 50ng miRNA from extracellular vesicles.
  • Sequencing recommendation – 5M reads*.

Illumina DNA Prep Library  – Uses Bead-Link transposome for whole genome sequencing. Broad application range including multi-species genomes, Microbial genomes,amplicons, plasmids. Unique Dual Index (UDI) adapter compatible.

  • Targeted input of high MW gDNA: large genomes 100 - 500ng / small genomes 10 - 500ng
  • Sequencing recommendation – 30 X sequencing coverage*.

TruSeq ChIP-Seq Library – Used as an epigenetic analysis tool, chromatin immunoprecipitation (ChIP) sequencing libraries leverage next-generation sequencing (NGS) to determine the distribution and abundance of DNA bound protein targets of interest across the genome. Fragmented control input chromatin and immunoprecipitated chromatin are provided by the investigator. A gel image of the high MW chromatin and fragmented chromatin are required for project submission (fragmented size range 600bp – 100bp).

  • Targeted input of fragmented chromatin: control input – 30ng, immunoprecipitated chromatin – 30ng.
  • Sequencing recommendation:  20M – 40M reads* determined by predicted genome distribution of target protein.
  • Sequencing recommendation for input chromatin – 45M reads.

Illumina DNA Prep for Enrichment – Used for targeted resequencing in human applications. Indexed DNA libraries are prepared for pooled enrichment using exome capture probes targeting 45Mb of coding sequence from >98% of RefSeq, CCDS and Ensemble coding content. Open enrichment compatibility allows the use of non-Illumina capture probe sets allowing the expansion of the species applications available for targeted exome sequencing. Unique Dual Index (UDI) adapter compatible.

  • Targeted input of intact, high MW gDNA - ≥100ng.
  • Sequencing recommendation – 50M 100bp paired end reads* for human, 50X coverage (4Gb of data).

Ovation RRBS Methyl-seq Library w/ oxBS Module  – Reduced Representation Bisulfite Sequencing (RRBS) is used to generate single base resolution DNA methylation information across a genomic sample. By analyzing a reduced representation of the genome, the amount of sequencing required is greatly reduced relative to whole genome bisulfite sequencing (WGBS). The TrueMethyl oxBS module allows the differential interrogation of both 5-hydroxymethylcytosine (5hmC) and 5-methylcytosine (5mC) and provides a method to accurately quantify the true level of cytosine methylation through the subtractive analysis of two parallel library sequence data sets, one with oxidative conversion of 5hmC and one without. 

  • Targeted input – 100ng RNase treated high MW gDNA.
  • Sequencing recommendation – 55M 100bp paired end reads*.
  • Determination of 5mC and 5hMC modified cytosine requires two independent library interrogations per sample.

*Read counts and X coverage can be modified to meet specific project demands.

Genome Sequencing Facility

University of Kansas Medical Center
Genome Sequencing Facility
1015 HLSIC MS 3028
2146 West 39th Street
Kansas City, KS 66160-7421
Phone: 913-588-7127
Fax: 913-588-7131