Learn about the equipment available through the KC-MORE Metabolism Core.
The EchoMRI TM 1100 Analyzer delivers precise body composition measurements of fat, lean, free water and total water masses in tissues and live animals weighing from 1 to 1100 grams. Scanning takes less than 2 minutes, and anesthesia is not required.
We have two Promethion Indirect Calorimetry systems that provide metabolic and behavioral measures for up to 16 mice on one of the systems and 8 rats or 8 mice at one time on the other. Metabolism and behavior are monitored continuously on a second-to-second basis and macros are provided for data analysis. Outcome measures include oxygen consumption (energy expenditure) carbon dioxide production, respiratory exchange ratio, ambulatory activity, sleep duration, food intake, water intake. The system also has running wheels if needed.
A 12- 24 cage BioDAQ system was acquired through a KU School of Medicine Research Equipment and Resource Procurement Award (ERPA).
The BioDAQ Food and Liquid Intake Monitor (BioDAQ) is a home cage-based instrument that automatically collects and records an animal's native feeding and drinking behavior. The singly housed animal can live chronically in the BioDAQ. Feeding and drinking modules are adapted to standard laboratory cages. Liquid preference testing can also be performed. Cages and hardware can be cleaned using standard animal facility procedures.
Solace Zone thermoneutral mouse caging racks are available for use in either vivarium. The core maintains four independent sets of racks each capable of holding 32 mouse cages that can be utilized at a density equivalent to other vivarium caging. Temperature is variable in increments of 5 degrees. The caging can be relocated to your main housing room to facilitate your needs and provide minimal disruption to your colony.
The respirometry treadmills enable cardiorespiratory fitness tests analogous to human fitness tests. These include sub-maximal and maximal tests to assess VO2max. Tests may be single or multistage. Short test duration enables many tests to be completed per day. The high-performance gas analyzers resolve rapid respirometry changes to quantify the degree of change in response to changing speed or incline (including changes in fuel utilization). Semi-quantitative assessment of fuel utilization is determined by the respiratory exchange ratio.
Quantitative fuel utilization is possible when the Promethion Core metabolic system is coupled to our Stable Isotope Analyzer. This analyzer enables the quantitation of the catabolism of specific fuels (such as glucose, fructose and palmitic acid) in addition to the standard respirometry measurements.
A Parr Instrument Bomb Calorimeter is available for measuring the calorie content of various sample types. The calorimeter measures the heat released from combusting the biomass and is useful in understanding metabolic processing.
Mitochondrial energy metabolism is studied with the O2k in many research areas including exercise physiology, aging, cancer, diabetes, neurodegeneration, oxidative stress, and medicine. With the development of High-Resolution FluoRespirometry (HRFR), HRFR combines measurement and control of oxygen concentration and respiratory oxygen flux with monitoring ROS production, mitochondrial membrane potential, ATP production, Ca2+, pH, and NO – all in one chamber.
The Horiba FluoroMax® 4 spectrometer is a powerful tool for measuring fluorescence of solids, liquids, powders and thin films. The instrument is easy to use and is controlled by FluorEssenceTM software with Origin® embedded for sophisticated data analysis.
Fluorescence polarization is a general method for measuring ligand-binding to proteins and nucleic acids, and also measure membrane micro-viscosity.
This technique can determine binding constants, concentrations of hormones and drugs in biological fluids, and provide information regarding structural features and changes in macromolecules such as proteins. Our polarizers are auto-calibrating, internal to the FluoroMax®-4, and completely automatic, under software control.